Table 2.

Column A shows the amounts of receptors (B_max) in membrane preparations from COS-7 and Neuro2A cells after transient transfection with the wt α_1D-adrenoceptor (wt), and with the signal peptide-supplemented α_1D-adrenoceptor (SP)

Transient expression in COS-7 or Neuro2A cells of wt and signal peptide-supplemented α1D-AR	A. Bmax receptors (fmol mg−1 protein)	B. Bmax receptors (% compared to wt)	C. pKd [3H]-prazosin	n
α1D-AR (wt) COS-7	69.3±6.4	100	9.93±0.10	4
α1D-AR (SP) COS-7	838±200	1190±220	9.83±0.07	4
α1D-AR (wt) Neuro2A	103±31	100	10.13±0.07	4
α1D-AR (SP) Neuro2A	1120±80	1300±240	9.90±0.09	4

Column B shows B_max of the signal peptide-supplemented α_1D-AR (SP) in percent of that of the wild type α_1D-AR (wt), from experimentally paired plates. Column C shows the pK_d values of [³H]-prazosin for the receptors.