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. 2005 Jan 24;144(5):715–726. doi: 10.1038/sj.bjp.0706114

Figure 5.

Figure 5

Effects of SV and nimodipine on Rho activation in rat aorta stimulated with either ET-1 or KCl. The endothelium-denuded rat aorta preparations were stimulated with either ET-1 (10 nM) or KCl (60 mM), and the muscle was snap frozen after 5 and 30 min of stimulation. SV (2 μM) or nimodipine (0.1 μM) were administered 10 min before the administration of ET or KCl. Rho activation was determined by using an affinity precipitation assay with GST-fusion protein of the RBD of the Rho effector rhotekin, as described in Methods. Panel a shows the effects of SV (2 μM) and nimodipine (0.1 μM) on Rho activation in rat aorta stimulated for 5 min (left) and 30 min (right) with ET-1 (10 nM). Panel b shows the effects of SV and nimodipine on Rho activation in rat aorta stimulated with KCl (60 mM) for 5 min (left) and 30 min (right). The position of GTP-Rho is indicated on the right of the representative immunoblots. Quantification of intensities of GTP-Rho bands was performed by scanning densitometry. Results are expressed as a percentage of respective controls, and are means±s.e.m. of duplicate determinations from five experiments.