Figure 8.

Effects of SV, GGTI-297 and HA-1077 on [³H]thymidine incorporation stimulated by ET-1 in VSM cells. [³H]thymidine incorporation was used to assess DNA synthesis. Subconfluent, quiescent cells were incubated with either vehicle (0.3% DMSO) or ET-1 (10 nM) for 48 h. SV (0.1–10 μM) (panel a), was added to quiescent cells 24 h before administration of ET-1.GGTI-297 (200 nM) (panel b) and HA-1077 (1 μM) (panel c) were administered 30 min prior to addition of ET-1 (10 nM) for 48 h. Data represent the means±s.e.m. of six experiments performed in triplicate. ^***P<0.001 versus control; ^##P<0.01 and ^###P<0.001 versus ET-1.