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. 2005 Feb 14;144(7):982–993. doi: 10.1038/sj.bjp.0706127

Figure 5.

Figure 5

The N- and the C-terminus of CBP confer sensitivity to cyclosporin A treatment after membrane depolarization. The upper panel shows a scheme of full-length CBP, the N-terminus (N), the middle domain (M), and the C-terminus of CBP (C). The white boxes within CBP indicate various CBP regions. NR, nuclear receptor; HAT, histone acetyltransferase; C/H3, zinc finger 3. The luciferase reporter gene under the control of five GAL4-binding sites (5xGal4E1BLuc) was transfected into HIT cells together with expression vectors for GAL4 fused to the three CBP regions depicted in the upper panel. Left panel, basal transcriptional activities of the N-terminus (N), the middle domain (M), and the C-terminus (C) of CBP. Luciferase activity is expressed relative to the mean value measured in each experiment of the GAL4 DNA-binding domain. Values are means±s.e.m. (n=6). Right panel, cells were treated as indicated with KCl (45 mM), forskolin 10 μM (cAMP), and cyclosporin A 5 μM (CsA). Luciferase activity is expressed relative to the mean value in each experiment of the activity measured in the respective control. *P<0.01 versus without CsA, Student's t-test. Values are means±s.e.m. (n=6).