Skip to main content
. 2005 Feb 14;144(7):982–993. doi: 10.1038/sj.bjp.0706127

Figure 7.

Figure 7

Calcineurin mediates part of the membrane depolarization-induced transcriptional activity of the CBP C-terminus. (a) The overexpression of calcineurin enhances CBP C-terminus-dependent transcription. The luciferase reporter gene under control of five copies the GAL4-binding site (5xGal4E1BLuc) was transfected into HIT cells together with an expression vector for the GAL4 DNA-binding domain fused to the CBP C-terminus (aa 1678–2441) and expression vectors for calcineurin subunits A and B. The white box indicates the pCIP interaction domain of CBP (aa 2040–2170). Cells were treated with KCl (45 mM) as indicated. Luciferase activity is expressed relative to the mean value in each experiment of the activity measured in the control without calcineurin. Values are means±s.e.m. (n=6). (b) Cyclosporin A and tacrolimus inhibit the transcriptional activity of the CBP C-terminus after membrane depolarization with high potency. The luciferase reporter gene under control of five copies the GAL4-binding site (5xGal4E1BLuc) was transfected together with an expression vector for the GAL4 DNA-binding domain fused to the CBP C-terminus (aa 1678–2441). Cells were stimulated by membrane depolarization (KCl 45 mM) and treated with increasing concentrations of cyclosporin A (CsA) or tacrolimus (FK506). Luciferase activity is expressed relative to the mean value in each experiment of the stimulus-induced activity in controls (no CsA, no FK506). Values are means±s.e.m. (n=6).