Figure 3.
Effect of prenatal cocaine exposure on expression of Bcl-2 in neonatal rat hearts. Hearts were obtained from 1 (D1)-, 3 (D3)-, 7 (D7)-, 21 (D21)-, and 30 (D30)-day-old neonates that were exposed to either saline control (C) or cocaine (30 mg kg−1 day−1) (E) before birth from days 15 to 21 of gestational age. Protein levels of Bcl-2 were determined using Western blot analyses. Proteins were visualized with an enhanced chemiluminescence detection system, and were quantified with KODAK Electrophoresis Documentation and Analysis System and KODAK 1D Image Analysis Software. Data are mean±s.e.m. *P<0.05, cocaine vs control, n=4–7.