Figure 4.

CBD-induced cell migration is not prevented by pretreatment with PTX. Cells were pretreated with 100 ng ml⁻¹ PTX for 4 h, then treated with CBD or vehicle B for 30 min (open columns) and loaded in the upper compartment of the Boyden chamber. Black columns represent cells without PTX treatment, with CBD or vehicle A alone. Results are expressed as percentages of control migration (U87 cell migration in the presence of vehicle). Values are mean±s.e.m. of at least three independent experiments. ^*P<0.05, ^**P<0.01 vs vehicle B; °P<0.05, °°P<0.01 vs vehicle A Tukey's t-test.