Figure 5.

Recording from an inside-out patch showing inhibition of the Kir6.2/SUR2A(YS) channel by GBC. After excision of the patch into nucleotide-free solution, a current was present, which was abolished by superfusion with MgATP (1 mM) and showed some run-down. MgATP was applied repeatedly to induce refreshment of channels from run-down and GBC intermittently as indicated by the hatched bars. Holding potential was −50 mV; experiments were performed in symmetrical high K⁺ buffer at 23°C. Data were corrected manually for run-down and for weakening of the MgATP block. Note the slow washout of GBC (1000 nM).