Table 2.
[3H]GBC binding to SUR2A(YS) as determined in saturation binding experiments
| Receptor | MgATP (mM) | KD (nM) | BMAX (fmol mg−1) |
|---|---|---|---|
| SUR2A(YS) | 0 | 5.6 (3.9, 8.1) | 1144±128 |
| 1 | 6.6 (4.5, 10) | 626±107a | |
| Kir6.2/SUR2A(YS) | 0 | 1.9 (1.1, 2.7) | 772±102 |
| 1 | 1.2 (0.9, 1.5) | 569±48a | |
| 0b | 0.88 (0.76, 1.0)b | 463±12b | |
| Cells | 0.53 (0.40, 0.69) | 310±23 | |
| Kir6.1/SUR2A(YS) | Cells | 0.75 (0.66, 0.87)c | 180±24 |
The respective experiments in the absence and presence of MgATP were carried out side by side using the same membrane preparation(s). Parameters were determined from experiments as shown in Figure 2.
a
Different from the value in the absence of MgATP (P<0.01 and <0.05, respectively).
b
Experiments at 23°C; incubation time was 45 min.
c
Different from Kir6.2/SUR2A(YS) (P<0.05).