Table 1.

Primer sequence for RT–PCR of muscarinic receptor subtypes and β-actin

Code	Primer sequence (5′ → 3′)	GenBank accession no.	Sequence position	Fragment size (bp)
M1	Sense: GCTCCCCAAATACAGTCAAGAG	NM_000738	1363–1384	338
	Antisense: CAGCAGCAGGCGAAAGGTGT		1700–1681
M2	Sense: GATGGCCTGGAGCACAACA	NM_000739	757–775	358
	Antisense: GCTGCTTAGTCATCTTCACAATC		1114–1092
M3	Sense: CGAGCAGATGGACCAAGAC	NM_000740	972–990	390
	Antisense: AGGTAGAGTGGCCGTGCTC		1365–1347
M4	Sense: TCCAATGAGTCCAGCTCAGG	NM_000741	874–893	488
	Antisense: AGAGCATAGCAGGCAGGGTTG		1361–1341
Nested M4	Sense: TCCAGATTGTGACGAAGCAG	NM_000741	1022–1041	202
	Antisense: AAGGCTAGCAGAATGGCAAA		1204–1223
M5	Sense: GGACTATAAGTTCCGATTGGTG	NM_012125	1445–1466	295
	Antisense: GGTGACTGGGACACACTTG		1739–1721
β-Actin	Sense: ACGGGGTCACCCACACTGTGC	NM_001101	543–563	659
	Antisense: CTAGAAGCATTTGCGGTGGAC		1201–1181