Figure 3.

Saturation isotherms of [³H]MPEP binding analysis performed on living HEK293 cells transiently transfected with the hmGlu5a WT or cysteine to serine mutants as indicated. Cells were plated at a density of 25 × 10⁴ cells per well. The average difference in DPM between total binding and nonspecific binding (defined by 10 μM MPEP) was then expressed as a percentage of that obtained for the WT (11 122.3±622.2DPM). Each point represents the mean±s.e.m. of at least three independent transfections and experiment performed in quadruplicates.