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. 2005 Feb 7;144(8):1118–1125. doi: 10.1038/sj.bjp.0706152

Figure 4.

Figure 4

Increased quisqualate-induced IP accumulation after inverse agonist pretreatment of hmGlu5a receptor. At 24 h post-transfection, HEK293 cells expressing the WT hmGlu5a or C57/99S mutant (each cotransfected with rEAAC1 and the Gαq subunit) were split into equal parts: the first parts (WT and C57/99S) received no special treatment and the second parts (WT+MPEP and C57/99S+MPEP) were incubated in the presence of 1 μM MPEP during 24 h before measuring the IP turnover as described in Methods. Extensive washing steps ensured that MPEP traces were removed from the assay medium prior to agonist stimulation. Each curve represents the mean±s.e.m. of three (WT) or four (C57/99S) dose–response measurements performed in quadruplicates from three or four independent transfections, respectively. MPEP-treated WT cells displayed more than 2.5-fold increase in efficacy as compared to nontreated cells.