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Effect of GDP on the inhibition of basal [35S]GTPγS binding by (+)-butaclamol. Inhibition of [35S]GTPγS binding by different concentrations of (+)-butaclamol was determined in the presence of different concentrations of GDP as described in Methods section. The data were fitted using a sigmoidal dose–response curve with a Hill coefficient of one. Curves are representative examples of data replicated as in Table 3.
