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. 2005 Feb 21;145(1):24–33. doi: 10.1038/sj.bjp.0706112

Figure 6.

Figure 6

Expression of rMCP-5 in granulomatous tissue. (a) RT–PCR assay of rMCP-5 mRNA levels in animals injected with saline solution or with (1%) λ-carrageenin. The expression of β-actin, a housekeeping gene, was used as a control of cDNA quantity. PCR was allowed to proceed for 30, 35, and 40 cycles using rMCP5 primers and for 10, 15, and 20 cycles using β-actin primers. The results demonstrate a linear relationship along the cycles. (b) Quantitative analysis of the results of three independent experiments expressed in arbitrary units after normalization to β-actin mRNA levels.