Table 1.

Effect of GW 5074 (20 nM), PD 98059 (10 μM), SB 203580 (10 μM), EGCG (20 μM) and EGC (20 μM) on NHE activity after short- (0.5 h) and long-term (24 h) exposure to IFN-γ (1000 U ml⁻¹) in Caco-2 cells

	Short-term	Long-term
Vehicle	100±7	100±9
IFN-γ	69±7*	57±3*
GW 5074	100±9	100±8
GW 5074+IFN-γ	112±7	99±7
Vehicle	100±7	100±10
IFN-γ	57±4*	64±9*
PD 98059	100±14	100±6
PD 98059+IFN-γ	95±9	104±6
Vehicle	100±10	100±13
IFN-γ	64±9*	58±2*
SB 203580	100±8	100±5
SB 203580+IFN-γ	116±4	114±10
Vehicle	100±9	100±13
IFN-γ	52±6*	58±2*
EGCG	100±12	100±10
EGCG+IFN-γ	62±8*	103±12
EGC	100±8	100±11
EGC+IFN-γ	68±4*	52±5*

Values indicate means of 12 experiments per group.

^*P<0.05, values significantly different from control values using the Student's t-test. The NHE activity (in pH units s⁻¹) in vehicle-treated cells in the short- and long-term stimulation series was 0.0033±0.0002 and 0.0050±0.0003, respectively.