Figure 6.

Effects of cyclosporine A on mahanine-induced morphological changes and apoptosis in U937 cells. U937 cells, with or without preincubation with cyclosporine A, were incubated with mahanine for 10 h. After incubation, cell morphology was examined by light microscopy (a); DNA content was measured by FACS after staining DNA with propidium iodide (b). The results are from a representative example of the three independent experiments performed. (c) Cells were preincubated with cyclosporine A and a general caspase inhibitor for 1 h. Following incubation with 10 μM mahanine for 10 h, DNA breaks in U937 cells were determined using the TUNEL assay and FACS. Control cells were incubated with media alone. One representative experiment of the three performed is shown.