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. 2005 Mar 7;145(2):228–235. doi: 10.1038/sj.bjp.0706183

Figure 2.

Figure 2

Effect of JMV1195 and JMV2764 on JMV2236-hydrolyzing activity by endogenous β-secretase or BACE1. Kinetics of β-secretase activities were measured with 10 μM of JMV2236 in the absence (control) or in the presence of 10 μM of JMV1195, JMV2764 or a commercial inhibitor (CI) with protein homogenates from HEK293 cells harbouring endogenous β-secretase (a) or overexpressed BACE1 (c). In panel c (inset) BACE1-like immunoreactivity in the indicated cell line was detected by means of anti-1D4 antibodies. In panels b and d, histograms compare the extent of inhibition of JMV2236-hydrolyzing activities triggered by 10 μM of indicated inhibitor after 30 min incubation with Mock- (b) or BACE1 (d)-transfected HEK293 cell extracts. Bars are the means±s.e.m. of four independent experiments.