Figure 5.

Effect of JMV2764 on Aβ production by wild-type βAPP expressing cells. Wild-type βAPP-expressing HEK293 cells were incubated for 6 h at 37°C with various concentrations of JMV2764. Then Aβ40 was immunoprecipitated with FCA3340 and detected by Western blot with WO2 as described in Methods (a). Cells were extracted and assayed for βAPP-like immunoreactivity with 10D5 as described in Methods. Bars in panel b represent the densitometric analysis of Aβ40 immunoreactivity expressed as the control production obtained in absence of inhibitor and are the means±s.e.m. of five experiments.