Figure 6.

Comparison of JMV2764 and JMV1195 on Aβ production by wild-type and Swedish-mutated βAPP-expressing cells. Wild-type (a,b) and Swedish-mutated (c,d) βAPP-expressing HEK293 cells were incubated for 6 h at 37°C with 10 or 100 μM of JMV1195 or JMV2764. Then Aβ40 was immunoprecipitated with FCA3340 and detected by Western blot with WO2 as described in Methods (a,c). Cells were extracted and assayed for βAPP-like immunoreactivity with 10D5 as described in Methods. Bars in panels b and d represent the densitometric analysis of Aβ40 immunoreactivity expressed as the control Aβ production obtained in absence of inhibitor and are the means±s.e.m. of four experiments.