Figure 7.

JMV2764 does not affect Aβ40 production by SPA4CT-expressing HEK293 cells. Aβ production from βAPP derives from subsequent cleavages by β- and γ-secretases (a) while a single cleavage by γ-secretase on SPA4CT releases an Aβ bearing a Leu-Glu N-terminal extension (LEAβ) (b). Stably transfected cells expressing βAPP produce a β-secretase-derived C99 fragment of slightly lower molecular weight than LEC99 (c). Aβ40 and Aβ42 species derived from SPA4CT-expressing cells were detected by FCA3340 and FCA3542, respectively, but not detected by a anti-N-terminal antibody (FCA18; Barelli et al., 1997) that recognizes only Asp1 residue of Aβ (d). Neither JMV1195 nor JMV2764 affect Aβ40 recovery from SPA4CT-expressing cells (e).