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. 2005 Mar 14;145(2):156–165. doi: 10.1038/sj.bjp.0706191

Figure 2.

Figure 2

Effect of PLC inhibition or PtdIns(4,5)P2 addition on the progressive decline of IK,ACh during exposure to ATP. (a, c) Time course of changes in the holding current level at −40 mV during ∼2.5 min exposure to ATP at concentrations of 10 μM (a) and 50 μM (c) in atrial myocyte dialyzed with a pipette solution containing either 100 μM compound 48/80 (a) or 50 μM PtdIns(4,5)P2 (c). The myocyte was stimulated with extracellular ATP 15–20 min after rupture of the patch membrane (establishment of whole-cell mode) with each reagent. (b, d) Percentage decrease of IK,ACh during exposure to ATP at 10 μM (b) and 50 μM (d) in myocytes dialyzed with control pipette solution (Control) and pipette solution supplemented with either 100 μM compound 48/80 (b) or 50 μM PtdIns(4,5)P2 (d). Percentage decrease was determined at the current levels measured at the end of ∼2.5 min exposure to ATP. Inset in (a) shows a representative time course of IK,ACh (at −40 mV) during exposure to 10 μM ATP, recorded from an atrial myocyte dialyzed with a control pipette solution. *P<0.05 and **P<0.01 compared with control value (Student's unpaired t-test).