Skip to main content
. 2005 Mar 21;145(3):283–292. doi: 10.1038/sj.bjp.0706200

Figure 5.

Figure 5

cGMP-mediated megakaryocyte apoptosis. (a) Megakaryocytes were treated with 8-pCPT-cGMP (n=3 in duplicate). (b) Cells were stimulated with BAY 41-2272 (n=5). ODQ (500 μM) was added 30 min before stimulation (n=3 in duplicate). (c) Apoptosis (black bars) was induced by PAPA/NO (100 μM), and acetylated cGMP (white bars) in megakaryocyte lysates was measured 15 min after NO addition. ODQ (500 μM) was added 30 min before the NO donor (n=5). (d) Cells were preincubated in the presence (white bars) or absence (black bars) of Zaprinast (40 μM) for 5 min before PAPA/NO and apoptosis was measured by flow cytometry (n=6). *P<0.05 vs none; #P<0.05 vs PAPA/NO; &P<0.05 vs Zaprinast alone.