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Inhibition of caspase-3 cleavage by cAMP raising agents. Megakaryocytes were treated for 1 min with PGI2 (3 μM) and further incubated for 5 h with PAPA/NO (100 μM). Activated caspase-3 was then detected by flow cytometry. Histograms show isotype (gray area) or activated caspase-3-stained cells (black line) and are representative of three similar experiments performed in duplicate.
