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Regulation of α-thrombin-stimulated VWF release or P-selectin expression. Megakaryocytes were incubated with PGI2 (3 μM) and/or PAPA/NO (100 μM) for 1 min and α-thrombin was then added as indicated. Incubation was continued for 30 min and supernatants (a) or cells (b) collected for VWF assay or P-selectin detection, respectively (n=5 in duplicate). *P<0.05 vs none; #P<0.05 vs α-thrombin alone; ¶P<0.05 vs PGI2+α-thrombin and NO+α-thrombin.
