Table 3.
Treatments | cAMP (nM) | cGMP (nM) |
---|---|---|
None | 0.29±0.01 | 0.10±0.01 |
PAPA/NO | 0.49±0.01* | 4.03±0.09* |
PGI2 | 2.68±0.08* | Nondetectable |
PGI2+PAPA/NO | 5.01±0.08¶,# | 0.60±0.02# |
Acetylated cAMP or cGMP in megakaryocyte lysates were determined 15 min post treatment with PGI2 (3 μM), PAPA/NO (100 μM) or the combination of both drugs (PGI2 was added 1 min before NO) (n=4 in duplicate).
P<0.05 vs none.
P<0.05 vs PGI2 alone.
P<0.05 vs PAPA/NO. The detection limit of the cGMP assay was 0.025 nM.