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. 2005 Mar 21;145(3):283–292. doi: 10.1038/sj.bjp.0706200

Table 3.

Regulation of intracellular cAMP and cGMP levels

Treatments cAMP (nM) cGMP (nM)
None 0.29±0.01 0.10±0.01
PAPA/NO 0.49±0.01* 4.03±0.09*
PGI2 2.68±0.08* Nondetectable
PGI2+PAPA/NO 5.01±0.08¶,# 0.60±0.02#

Acetylated cAMP or cGMP in megakaryocyte lysates were determined 15 min post treatment with PGI2 (3 μM), PAPA/NO (100 μM) or the combination of both drugs (PGI2 was added 1 min before NO) (n=4 in duplicate).

*

P<0.05 vs none.

P<0.05 vs PGI2 alone.

#

P<0.05 vs PAPA/NO. The detection limit of the cGMP assay was 0.025 nM.