Figure 4.

2-APB has no effect when applied to the intracellular face of the membrane. (a) Typical inside-out patch currents at −80 and +80 mV recorded from a Tet+ cell and showing TRPC5 current in response to GTP-γ-S. (b) In the presence of 10 μM Gd³⁺ in the pipette (mean±s.e.m., n=7) bath-applied 2-APB (75 μM) had no significant effect. (c) Mean±s.e.m. inside-out patch currents for Tet+ (n=7, P>0.05 for control vs 2-APB) and Tet− cells (n=8, P>0.05 for control vs 2-APB). (d) Typical whole-cell current recorded from a Tet+ cell with 150 μM 2-APB in the patch pipette and 10 μM Gd³⁺ in the bath. The x-axis is the time after breaking the cell-attached patch to form the whole-cell. At 33 min, 2-APB (75 μM) was applied to the bath solution.