Figure 7.

Resistance of TRPM2 to 2-APB. HEK cells were uninduced (Tet−) or induced to express TRPM2 (Tet+). (a) TRPM2 current was induced following break-through to the whole-cell (WC) with the 200 nM Ca²⁺ pipette solution containing ADP-ribose (0.5 mM). Replacement of Na⁺ with NMDG inhibited inward current. IV curves are inset. Typical of three experiments. (b) Current amplitudes at +80 and −80 mV in a TRPM2 cell and showing the lack of effect of bath-applied 2-APB at 75 and 150 μM. (c) IV curves from the experiment shown in (b). (d) Mean±s.e.m. current amplitudes at −80 mV for TRPM2-expressing (Tet+) cells (n=7) and non-induced (Tet−) cells showing endogenous current (n=3). ^***P<0.001.