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. 2005 Apr 11;145(4):477–489. doi: 10.1038/sj.bjp.0706210

Figure 1.

Figure 1

Time-dependent phosphorylation of (a) GST-MARCKS by the PKC isoforms PKCα and PKCɛ and (b) GST-NHE1 by the p90RSK isoforms RSK1, RSK2 and RSK3. Recombinant human PKCα and PKCɛ were incubated with GST-MARCKS for 0–60 min at 37°C, prior to addition of SDS–PAGE sample buffer and Western immunoblot analysis with an antibody recognising pS152/pS156 of MARCKS. Similarly, recombinant human RSK1, RSK2 and RSK3 were incubated with GST-NHE1 for 0–60 min at 37°C, prior to addition of SDS–PAGE sample buffer and Western immunoblot analysis with an antibody recognising the RXRXX(pS) motif in GST-NHE1. An antibody recognising GST was used to confirm the presence of equal amounts of substrate. Autoradiograms representative of three experiments.