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. 2005 Apr 11;145(4):477–489. doi: 10.1038/sj.bjp.0706210

Figure 6.

Figure 6

Effects of GF109203X on the phosphorylation status of ERK1/2, p90RSK, eEF2K and eEF2 following adenoviral expression of caMEK1. ARVM were maintained in culture for 42 h, following a 1 h infection with empty virus (Cont) or adenovirus encoding caMEK1, both at an MOI of 50 PFU cell−1. ARVM were then exposed to vehicle (Veh), 1 μM U0126 (UO) or 1–10 μM GF109203X (GF) for 4 h, before being lysed in SDS–PAGE sample buffer for subsequent Western immunoblot analysis. (a) Representative Western immunoblots showing the expression of MEK1 and phosphorylated forms of ERK1/2 (P-ERK1/2), p90RSK(P-p90RSK), eEF2K (P-eEF2K) and eEF2 (P-eEF2). Total ERK2 expression is also shown to illustrate equal protein loading. Quantitative data (panels b–e) illustrate the phosphorylation status of (b) ERK1/2, (c) p90RSK, (d) eEF2K and (e) eEF2, *P<0.05 versus Cont, †P<0.05 versus Veh (n=6).