Figure 5.
Effect of membrane potential on ethanol inhibition of ATP-activated current for the H241A mutant of rat P2X4 receptors expressed in Xenopus oocytes. Current–voltage relationship (I–V plot) showing the average amplitude of current activated by 6 μM ATP as a function of membrane potential, in the absence and presence of 50 mM EtOH (n=5). Note that EtOH did not alter the reversal potential of ATP-activated current (0±3 mV in the absence vs −1±2 mV in the presence of 50 mM ethanol; Student's t-test, P>0.05, n=5). The percentage inhibition of ATP-activated current by 50 mM EtOH was also not significantly different at the holding potentials shown (ANOVA, P>0.05, n=5).