Table 1.
Influence of various experimental conditions and receptor antagonists on changes in diastolic blood pressure (DBP) induced by anandamide (3 or 9 μmol kg−1). All results were compared to the anandamide-induced triphasic changes in blood pressure found in urethane-anaesthetized rats
| Experimental condition | Antagonist | Dose(s) (μmol kg−1) | Influence on DBP | ||
|---|---|---|---|---|---|
| Rats anaesthetized with | Phase I | Phase II | Phase III | ||
| Urethane | SR 141716Aa | 3 | NO | NO | Inhibition |
| Propranolol | 0.3 | NO | Inhibition | NO | |
| ICI 118551 | 1 | NO | Inhibition | NO | |
| CGP 20712 | 1 | NO | NO | NO | |
| Prazosinb | 1 | NE | NO | NO | |
| Rauwolscineb | 1 | NE | NO | NO | |
| Guanethidineb | 2 × 125 | NE | NO | Inhibition | |
| BIBP3226b | 3 | NO | NO | NO | |
| Suramin | 300 | NO | NO | NO | |
| Ruthenium red | 3 | Inhibitiona | Inhibition | NO | |
| Capsazepine | 1 | Inhibitiona | NO | NO | |
| Nifedipineb | 1 | NO | Inhibition | NO | |
| MK-801 | 1 | NO | Inhibition | NO | |
| Captoprilb | 10 | NE | NO | NO | |
| Losartanb | 10 | NO | NO | NE | |
| Ketanserin | 0.09 | NE | NO | NO | |
| Tezosentan | 10 | NO | NO | NO | |
| VP-antagonist | 0.01 | NO | NO | NO | |
| Urethane and vagotomized | None | 0 | Inhibition | NO | NO |
| Urethane and adrenalectomized | None | 0 | NO | NO | NO |
| Urethane, pithed and vagotomized | Noneb | 0 | Inhibition | NO | Inhibition |
| Pentobarbitone | None | 0 | Inhibition | Inhibition | NO |
| Pentobarbitone, pithed and vagotomized | Noneb | 0 | Inhibition | Inhibition | Inhibition |
All antagonists were injected i.v. with the exception of guanethidine, which was administered i.p. in two doses 14 and 1 h before the experiment.
a
Data from Malinowska et al. (2001).
b
Experiments performed under infusion of vasopressin.
VP-antagonist, d(CH2)5[Tyr(Me)2Arg8]-vasopressin.
NE=not evaluated because of too low hypotensive response (lower than 10%).
NO=no influence on the effect of anandamide.