Table 3.
Effects of propiverine metabolites on the CRC for CCh in pig detrusor
| n | First CRC- | Second CRC | |||
|---|---|---|---|---|---|
| −log EC50 | −log EC50 | Δ−log EC50a | Effmax | ||
| TMC | 15 | 5.64±0.19 | 5.00±0.13 | 0.63±0.11 | 90±3 |
| M-5 | |||||
| 100 μM | 5 | 5.91±0.17 | 4.94±0.26 | 0.34±0.21 | 40±8*** |
| 300 μM | 4 | 5.99±0.14 | 5.02±0.16 | 0.34±0.08 | 14±4*** |
| M-6 | |||||
| 1 μM | 5 | 5.69±0.22 | 3.82±0.30 | 1.24±0.13*** | 85±13 |
| 100 μM | 4 | 5.97±0.10 | 1.44±0.06 | 3.89±0.15*** | 83±5 |
| M-14 | |||||
| 1 μM | 4 | 5.86±0.12 | 5.03±0.20 | 0.20±0.25 | 81±4 |
| 10 μM | 4 | 6.11±0.06 | 5.34±0.11 | 0.18±0.08 | 54±7*** |
| 30 μM | 2 | 5.99 | 5.44 | −0.09 | 10*** |
| 100 μM | 4 | 5.15±0.39 | 4.64±0.43 | −0.24±0.26 | 8±4*** |
| 300 μM | 3 | 6.06±0.14 | 5.08±0.30 | 0.35±0.21 | 5±1*** |
−log EC50 (M) and Effmax (% of the maximum response in the first CRC) was determined from curve fitting to the experimental data obtained in each individual muscle strip. Data as means±s.e.m., n=number of detrusor strips. Δ−log EC50 are the differences between first and second CRC (i.e. the shift of CRC to higher CCh concentrations) and were corrected for the mean shift for TMC (with exception for the TMC itself).
*
P<0.05;
**
P<0.01;
***
P<0.001.
a
Tested for difference from zero.