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Modulation of [3H]P1075 binding to SUR2A and SUR2B by PIP2. Data show specific binding (BS) and are means±s.e.m. from seven experiments each. Note the small but significant stimulation of [3H]P1075 binding to SUR2A at 30 μM PIP2. Experiments were performed in the presence of MgATP (0.3 mM) at [3H]P1075 concentrations of 3 (SUR2A) and 2 nM (SUR2B). 100% BS was 260–300 fmol mg−1 and nonspecific binding (BNS) was ∼10% of total binding (BTOT).
