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. 2005 May 23;145(8):1103–1111. doi: 10.1038/sj.bjp.0706280

Figure 4.

Figure 4

(a) Western blot analysis of cdk5, p35/p25 and p-MEF2. Evidence that cdk5 overexpression in CGNs after 12 h S/K withdrawal is blocked in the presence of 40 μM PD150606 or PD151746. The p35/p25 ratio was also evaluated, showing an increase in p25 band intensity after S/K deprivation and a return to control levels in the presence of PD150606 or PD151746. Representative results for 10 μM roscovitine are showed. Finally, MEF2 band intensity decreased when CGNs were S/K deprived, indicating inactivation of MEF2, and increased in the presence of PD150606 or PD151746. All experiments were carried out at least in duplicate on three different cultures. (b) Immunostaining against cdk5 and MEF2 of CGNs after 12 h S/K withdrawal in the absence or presence of 40 μM PD150606 demonstrated an increase in cdk5 expression after S/K withdrawal and an inactivation of prosurvival MEF. Similar results were obtained with PD151746 (data not shown) (scale bar, 25 μm).