Figure 6.

Effect of apamin (1 μM) on the inhibitory responses induced by EFS in the NANC conditions at different stimulation frequencies (0.5 ms, 50 V cm⁻¹ for 10 s). The magnitudes of the EFS-evoked responses are expressed as the % change in the amplitude (the amplitude of the inhibitory phase normalized to the amplitude of the muscle activity directly precedent to the inhibitory stimulus), and the values shown are mean±s.e. (a) Apamin significantly reduced the magnitude of EFS-induced inhibitions at all frequencies in Cav1^−/− mouse intestine. Significance was tested by ANOVA, followed by Bonferroni test (^*P<0.05; ***P<0.001, n-value=6). (b) Apamin did not produce a significant effect on the EFS-induced inhibitions in the Cav1^+/+ mouse intestine, except at 30 pps (^*P<0.05, n-value=6).