Table 2.
Characteristics of antibodies used for immunohistochemistry (ICC) and Western blotting
| Antibody | Type | Source | Dilutions | |
|---|---|---|---|---|
| ICC | Western | |||
| α-SMA | Monoclonal, IgG, mouse | 1 | 1/500 | 1/10.000 |
| Vimentin | Monoclonal, IgM, mouse | 1 | 1/500 | 1/10.000 |
| Desmin | Monoclonal, IgG, mouse | 1 | 1/500 | 1/10.000 |
| GFAP | Monoclonal, IgG, mouse | 1 | 1/500 | — |
| Synemin | Polyclonal, IgG, rabbit | 2 | 1/1000 | — |
| Nestin | Polyclonal, IgG, rabbit | 3 | 1/200 | 1/7.500 |
| Secondary AB | Anti-mouse, IgG, sheepa | 4 | 1/200 | 1/20.000 |
| Secondary AB | Anti-mouse, IgM, goata | 1 | 1/250 | 1/20.000 |
| Secondary AB | Anti-rabbit, IgG, Poly-HRP, goata | 5 | 1/1 | 1/20.000 |
a
Peroxidase conjugated.
1
Sigma, Bornem, Belgium;
2
Courtesy Dr Z.L. Li, Biologie Moléculaire de la différenciation, Université Denis-Diderot, Paris, France;
3
Courtesy Dr J. Eriksson, Turku Centre for Biotechnology, University of Turku, Finland;
4
Amersham Biosciences, Roosendaal, The Netherlands and 5Chemicon International, Temecula, CA, U.S.A.