Table 2.
Effects of antioxidants on NOS and COX activities, and proinflammatory cytokine levels induced by HHb in HPMCs
| Effector | NOS activity (NOx, μM) | COX activity (RLUs/103) | IL-6 (pg ml−1) | TNF-α (pg ml−1) | IL-1β (pg ml−1) |
|---|---|---|---|---|---|
| None | 3.8±2.1 | 7.5±2.4 | 1.9±0.2 | 53.9±6.2 | 4.3±2.8 |
| HHb | 14.0±1.8* | 26.9±11.1* | 5.8±2.6* | 120.0±4.0* | 12.3±3.6* |
| HHb+SOD | 4.9±0.1† | 16.7±3.8† | ND | ND | ND |
| HHb+Tempol | 5.3±0.3† | 10.8±1.1† | 2.8±0.1† | 81.0±10.1† | 6.0±1.0† |
| HHb+DMTU | 3.7±0.1† | 11.6±5.8† | ND | ND | ND |
ND=not determined.
The effectors were used at the following concentrations: HHb and NHb at 10 nM, SOD at 200 U ml−1, Tempol at 100 μM, and DMTU at 1 mM, n=9.
*
P⩽0.05 vs basal;
†
P⩽0.05 vs HHb-treated cells.