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. 2005 Jul 11;146(3):397–407. doi: 10.1038/sj.bjp.0706330

Figure 8.

Figure 8

(a–e) Effects of PARAP2 on extracellular ATP-induced Ca2+ mobilization in Calu-3 cells preloaded with fluo-3. Application of ATP (100 μM, apical) on the apical side increased [Ca2+]i in a oscillatory fashion (a). The ATP-induced Ca2+ mobilization, which was significantly suppressed by D609 (100 μM, b) or PMA (1 μM, c), was also abolished by pretreatment with the PAR2-activating peptide (PAR2AP, 50 μM, basolateral, d). The PAR2-mediated attenuation was blunted by pretreatment with the PKC inhibitor GF109203X (GFX, 1 μM, e). D609, PMA, PAR2AP, and GF109203X were applied 30 min before addition of ATP.