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Real time PCR assays of insulin effect on CYP7A1 and PEPCK mRNA expressions in human primary hepatocytes. Primary human hepatocytes (HH 1251) were treated with increasing concentrations of insulin for 2 h (A) or 6 h (B). CYP7A1 or PEPCK mRNA expression was assayed in triplicates and expressed as mean ± standard deviation.
