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. Author manuscript; available in PMC: 2007 Sep 29.
Published in final edited form as: J Biol Chem. 2006 Aug 2;281(39):28745–28754. doi: 10.1074/jbc.M605815200

Fig. 4.

Fig. 4

Effect of SREBP-1c on human CYP7A1 reporter activity. A. Human CYP7A1 reporter constructs (0.2μg) were co-transfected with pcDNA3 empty vector or SREBP-1c expression plasmids (0.1 μg) into HepG2 cells and luciferase activities were analyzed 40 hr after transfection. B. HNF4α site mutant reporter (mHNF4-ph1887/Luc) (0.2μg) was co-transfected with 0.1μg pcDNA3 or SREBP-1c expression plasmid into HepG2 cells. C. Mammalian one-hybrid assay. The Gal4/luciferase reporter, 5xUAS/TK/Luc (0.2μg) was co-transfected with Gal4 empty vector (−)(0.1μg), Gal4-HNF4α (0.1μg), PGC-1α (0.1μg) and increasing amounts of SREBP-1c expression plasmid (0.05-0.2μg) as indicated. D. Human CYP7A1 reporter construct, ph-371-Luc (0.2 μg) was co-transfected with 0.1 μg each of HNF4α and PGC-1α and/or SREBP-1c expression plasmid as indicated. Each assay was performed in triplicates and statistical analysis was performed using student’s t-test, “*”, significant, P< 0.05.