Fig. 5.
Western blotting analysis of FoxO1, SREBP-1c and HNF4α in primary human hepatocytes treated with insulin. Primary human hepatocytes were treated with 10 nM insulin for the time indicated. Total cell lysates or nuclei fractions from donor HH1286, HH1281 and HH1249 were used for detection of FoxO1 (A), SREBP-1c (B) and HNF4α (C) by antibodies, respectively.