Fig 2.

 NaB-induced activation of HSF. HT 29 cells were transiently cotransfected with the pHSE-Luc reporter plasmid and the pRL-CMV plasmid for control of transfection efficiency. After transfection, cells were further treated with 3 mM NaB for the time indicated. The luciferase activities resulting from the HSE activation were normalized and calculated as a ratio with cells that did not received NaB. The data presented are the averages of 3 separate experiments (mean ± SE). Statistical differences were determined by 1-way ANOVA. Once significance was reached, Dunnett's multiple comparison post hoc test was used to compare between 0 exposure time and other time points; * P < 0.05