Figure 5.

(A) Schematic representation of interplasmid transposition events. Transposition of a Minos transposon from the donor plasmid into the sucrase gene carried by the target plasmid results in a combined plasmid that can be selected by using a triple selection scheme (Chl, chloramphenicol; Tet, tetracycline; SacRB, sucrase). (B) Sequences flanking the ends of Minos insertions in the sucrase gene, in events that occurred in A. stephensi embryos. The nucleotide positions in the sucrase gene (Genbank accession nos. XO2730 and KO1987), at which the insertion took place are indicated. The orientation of the insertion relative to the sucrase sequence also is indicated (ML-MR or MR-ML).