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Effect of IL-18BP on IL-12-induced IFN-γ production in PBMC. PBMC from three donors were cultured in flat bottom wells at 5 × 106 cells/ml in RPMI and were incubated in the absence or presence of 0, 10, 50, 100, or 200 ng/ml of IL-12 (unbroken line). The broken line represents cultures incubated with IL-12 plus 100 ng/ml of IL-18BP. IFN-γ levels represent the fold-change ±SEM over that of the production in unstimulated cells (control, set at 1).
