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Effect of IL-18BP on PGE2 production in whole blood cultures. Whole blood was obtained from three donors, and 0.5 ml was aliquoted into 75-mm polypropylene tubes to which was added a suspension of S. epidermidis (0.5 ml of RPMI). After 24 hr at 37°C, the cultures were lysed in Triton X-100 (0.5%) and were assayed for PGE2. IL-18BPa His6-tag was added at the time of stimulation with S. epidermidis, and the concentrations (ng/ml) are indicated under the horizontal axis.
