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. 1986 Jan;63(1):49–57.

Chlamydial antibody crossreactivity with peripheral blood mononuclear cells of patients with ankylosing spondylitis: the role of HLA B27.

D Wakefield, J Easter, P Robinson, D Graham, R Penny
PMCID: PMC1577339  PMID: 3485486

Abstract

We have previously reported the association of Chlamydia trachomatis with HLA B27+ related diseases. To investigate the possibility that chlamydial antibodies serve to localize the immune response in such diseases, we examined the crossreactivity of chlamydial antibodies (rabbit anti-D and anti-L2 serotypes) with peripheral blood mononuclear cells of patients with ankylosing spondylitis (AS) and anterior uveitis (AU) and with human and bovine ocular tissue and cells in culture. Our results indicate a significantly increased percentage binding of chlamydial antibody (D serotype) to the mononuclear cells of HLA B27+ patients with AS when compared with HLA B27- patients with AS (12.9% +/- 2.2 versus 5.4% +/- 2.2), B27+ controls (5.5% +/- 1.5) and B27- controls (6.1% +/- 1.0). There was no significant difference between controls and HLA B27+ patients with AU (6.6% +/- 1.9) and B27- patients with AU (8.7% +/- 1.1). This crossreactivity could not be blocked by monoclonal HLA B27 antibody. Chlamydial antibodies (D and L2) crossreact with human and bovine conjunctiva but not uvea, tissue culture derived iris fibroblasts or smooth muscle cells. Our results provide additional support for the concept of crossreactivity between antibodies to microbial agents and peripheral blood mononuclear cells of patients with HLA B27+ AS.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amor B., Coste F., Delbarre F. Mise en évidence, par culture, d'agents du genre Bedsonia dans le liquide articulaire en cas de rhumatisme de Fiessinger-Leroy-Reiter (F. L. R.) C R Acad Sci Hebd Seances Acad Sci D. 1967 Mar 6;264(10):1365–1367. [PubMed] [Google Scholar]
  2. Avakian H., Abuknesha R., Welsh J., Ebringer A. Uveitis, vitreous humor, and klebsiella. I. Binding studies with rabbit antisera. Br J Ophthalmol. 1981 May;65(5):315–322. doi: 10.1136/bjo.65.5.315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brewerton D. A., Caffrey M., Nicholls A., Walters D., James D. C. Acute anterior uveitis and HL-A 27. Lancet. 1973 Nov 3;302(7836):994–996. doi: 10.1016/s0140-6736(73)91090-8. [DOI] [PubMed] [Google Scholar]
  4. Caldwell H. D., Kromhout J., Schachter J. Purification and partial characterization of the major outer membrane protein of Chlamydia trachomatis. Infect Immun. 1981 Mar;31(3):1161–1176. doi: 10.1128/iai.31.3.1161-1176.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ebringer R. W., Cawdell D. R., Cowling P., Ebringer A. Sequential studies in ankylosing spondylitis. Association of Klebsiella pneumoniae with active disease. Ann Rheum Dis. 1978 Apr;37(2):146–151. doi: 10.1136/ard.37.2.146. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ehlers N., Kissmeyer-Nielsen F., Kjerbye K. E., Lamm L. U. Letter: HL-A27 in acute and chronic uveitis. Lancet. 1974 Jan 19;1(7847):99–99. doi: 10.1016/s0140-6736(74)92330-7. [DOI] [PubMed] [Google Scholar]
  7. Geczy A. F., Alexander K., Bashir H. V., Edmonds J. A factor(s) in Klebsiella culture filtrates specifically modifies an HLA-B27 associated cell-surface component. Nature. 1980 Feb 21;283(5749):782–784. doi: 10.1038/283782a0. [DOI] [PubMed] [Google Scholar]
  8. Karr R. W., Hahn Y., Schwartz B. D. Structural identity of human histocompatibility leukocyte antigen-B27 molecules from patients with ankylosing spondylitis and normal individuals. J Clin Invest. 1982 Feb;69(2):443–450. doi: 10.1172/JCI110468. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Keat A. C., Thomas B. J., Taylor-Robinson D., Pegrum G. D., Maini R. N., Scott J. T. Evidence of Chlamydia trachomatis infection in sexually acquired reactive arthritis. Ann Rheum Dis. 1980 Oct;39(5):431–437. doi: 10.1136/ard.39.5.431. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mapstone R., Woodrow J. C. Letter: Acute anterior uveitis and HL-A 27. Lancet. 1974 Apr;1(7859):681–682. doi: 10.1016/s0140-6736(74)93235-8. [DOI] [PubMed] [Google Scholar]
  11. Mattila L., Granfors K., Toivanen A. Acute anterior uveitis after yersinia infection. Br J Ophthalmol. 1982 Mar;66(3):209–212. doi: 10.1136/bjo.66.3.209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ostler H. B., Schachter J., Dawson C. R. Ocular infection of rabbits with a Bedsonia isolated from a patient with Reiter's syndrome. Invest Ophthalmol. 1970 Apr;9(4):256–262. [PubMed] [Google Scholar]
  13. Saari K. M., Vilppula A., Lassus A., Leirisalo M., Saari R. Ocular inflammation in Reiter's disease after Salmonella enteritis. Am J Ophthalmol. 1980 Jul;90(1):63–68. doi: 10.1016/s0002-9394(14)75077-9. [DOI] [PubMed] [Google Scholar]
  14. Schachter J., Barnes M. G., Jones J. P., Jr, Engleman E. P., Meyer K. F. Isolation of bedsoniae from the joints of patients with Reiter's syndrome. Proc Soc Exp Biol Med. 1966 May;122(1):283–285. doi: 10.3181/00379727-122-31113. [DOI] [PubMed] [Google Scholar]
  15. Schachter J. Complement-fixing antibodies to Bedsonia in Reiter's syndrome, TRIC agent infection, and control groups. Am J Ophthalmol. 1971 Apr;71(4):857–860. doi: 10.1016/0002-9394(71)90255-8. [DOI] [PubMed] [Google Scholar]
  16. Schachter J., Grossman M. Chlamydial infections. Annu Rev Med. 1981;32:45–61. doi: 10.1146/annurev.me.32.020181.000401. [DOI] [PubMed] [Google Scholar]
  17. Seager K., Bashir H. V., Geczy A. F., Edmonds J., de Vere-Tyndall A. Evidence for a specific B27-associated cell surface marker on lymphocytes of patients with ankylosing spondylitis. Nature. 1979 Jan 4;277(5691):68–70. doi: 10.1038/277068a0. [DOI] [PubMed] [Google Scholar]
  18. TAVERNE J., BLYTH W. A., REEVE P. TOXICITY OF THE AGENTS OF TRACHOMA AND INCLUSION CONJUNCTIVITIS. J Gen Microbiol. 1964 Nov;37:271–275. doi: 10.1099/00221287-37-2-271. [DOI] [PubMed] [Google Scholar]
  19. Wakefield D., Easter J., Robinson P., Penny R. Immunological features of HLA-B27 anterior uveitis. Aust J Ophthalmol. 1983 Feb;11(1):15–19. [PubMed] [Google Scholar]
  20. Wakefield D., Penny R. Cell-mediated immune response to chlamydia in anterior uveitis: role of HLA B27. Clin Exp Immunol. 1983 Feb;51(2):191–196. [PMC free article] [PubMed] [Google Scholar]
  21. Wakefield D., Wright J., Penny R. HLA antigens in uveitis. Hum Immunol. 1983 Jun;7(2):89–93. doi: 10.1016/s0198-8859(83)80009-3. [DOI] [PubMed] [Google Scholar]
  22. Willshaw H. E. Acute anterior uveitis and Klebsiella aerogenes: a casual relationship? Br J Ophthalmol. 1981 Nov;65(11):796–797. doi: 10.1136/bjo.65.11.796. [DOI] [PMC free article] [PubMed] [Google Scholar]

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