Figure 5.

VE dietary supplementation reduced chromosomal damage. (A) Percentage of cells with chromosomal alterations. The histogram shows that the cumulative frequency of aberrant metaphases as measured by the presence of chromatid and chromosome gaps, and/or breaks, and/or exchanges was significantly lower in TGFα/c-myc mice fed with VE-supplemented diet (*P < 0.05). The cytogenetic analysis was performed on primary hepatocyte cultures established from 10-wk-old TGFα/c-myc mice fed with control or VE-supplemented diet as described in Materials and Methods. The 50–100 metaphase spreads were scored for each individual mouse according to the international nomenclature as described in Materials and Methods. Values are expressed as the mean percentage ± SE. (B and C) Representative examples of chromosomal aberrations in TGFα/c-myc mouse fed with control (B) or VE-supplemented diet (C). Note extensive breakage and chromosomal rearrangements in TGFα/c-myc mouse fed with control diet. Arrows indicate gaps, breaks, and triradial and quadroradial exchanges.