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In vitro replication of M001 in human glioma cells. Replicating monolayers of U251MG (Upper) or D54MG (Lower) human malignant glioma cell lines were infected at 1 pfu per cell with HSV-1 (F) (closed diamonds), R3659 (closed circles), or M001 (open circles). Replicate cultures were harvested at 12, 24, 48, and 72 h after infection, and virus titers were determined on Vero cell monolayers.
