Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1996 Mar;110(3):957–964. doi: 10.1104/pp.110.3.957

Fusicoccin Binding to Its Plasma Membrane Receptor and the Activation of the Plasma Membrane H+-ATPase (III. Is There a Direct Interaction between the Fusicoccin Receptor and the Plasma Membrane H+-ATPase?).

M I De Michelis 1, F Rasi-Caldogno 1, M C Pugliarello 1, C Olivari 1
PMCID: PMC157795  PMID: 12226232

Abstract

A radioimmunoassay using antibodies raised against bovine serum albumin-conjugated fusicoccin (FC) was applied to measure FC bound to the plasma membrane (PM) isolated from seedlings of radish (Raphanus sativus L.) and of Arabidopsis thaliana treated in vivo plus or minus the toxin. FC bound to the PM from seedlings treated with 5 [mu]M FC was 2-fold (radish) to 7-fold (A. thaliana) higher than the binding capacity of control PM. FC binding depended on the duration of the in vivo treatment but was unaffected by cycloheximide. When FC binding and the PM H+-ATPase activity were compared under different conditions (in vivo or in vitro treatment of different lengths or with different concentrations of FC), a strict linear relation between FC binding and the activation of the PM H+-ATPase was observed in both plant materials under all the conditions tested. Comparison between the maximum binding capacity and the amount of H+-ATPase observed in PM from the two plant materials suggest a one-to-one stoichiometry between the FC receptor and the PM H+-ATPase.

Full Text

The Full Text of this article is available as a PDF (855.5 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aducci P., Ballio A., Blein J. P., Fullone M. R., Rossignol M., Scalla R. Functional reconstitution of a proton-translocating system responsive to fusicoccin. Proc Natl Acad Sci U S A. 1988 Nov;85(21):7849–7851. doi: 10.1073/pnas.85.21.7849. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aducci P., Ballio A., Fogliano V., Fullone M. R., Marra M., Proietti N. Purification and photoaffinity labeling of fusicoccin receptors from maize. Eur J Biochem. 1993 May 15;214(1):339–345. doi: 10.1111/j.1432-1033.1993.tb17929.x. [DOI] [PubMed] [Google Scholar]
  3. Aitken A., Collinge D. B., van Heusden B. P., Isobe T., Roseboom P. H., Rosenfeld G., Soll J. 14-3-3 proteins: a highly conserved, widespread family of eukaryotic proteins. Trends Biochem Sci. 1992 Dec;17(12):498–501. doi: 10.1016/0968-0004(92)90339-b. [DOI] [PubMed] [Google Scholar]
  4. Anthon G. E., Spanswick R. M. Purification and properties of the h-translocating ATPase from the plasma membrane of tomato roots. Plant Physiol. 1986 Aug;81(4):1080–1085. doi: 10.1104/pp.81.4.1080. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Briskin D. P., Reynolds-Niesman I. Change in Target Molecular Size of the Red Beet Plasma Membrane ATPase during Solubilization and Reconstitution. Plant Physiol. 1989 Jun;90(2):394–397. doi: 10.1104/pp.90.2.394. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. De Michelis M. I., Pugliarello M. C., Rasi-Caldogno F. Fusicoccin Binding to Its Plasma Membrane Receptor and the Activation of the Plasma Membrane H-ATPase: I. Characteristics and Intracellular Localization of the Fusicoccin Receptor in Microsomes from Radish Seedlings. Plant Physiol. 1989 May;90(1):133–139. doi: 10.1104/pp.90.1.133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Feyerabend M., Weiler E. W. Monoclonal antibodies against fusicoccin with binding characteristics similar to the putative fusicoccin receptor of higher plants. Plant Physiol. 1987 Nov;85(3):835–840. doi: 10.1104/pp.85.3.835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Johansson F., Sommarin M., Larsson C. Fusicoccin Activates the Plasma Membrane H+-ATPase by a Mechanism Involving the C-Terminal Inhibitory Domain. Plant Cell. 1993 Mar;5(3):321–327. doi: 10.1105/tpc.5.3.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Korthout H. A., de Boer A. H. A fusicoccin binding protein belongs to the family of 14-3-3 brain protein homologs. Plant Cell. 1994 Nov;6(11):1681–1692. doi: 10.1105/tpc.6.11.1681. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Korthout HAAJ., Van Der Hoeven PCJ., Wagner M. J., Van Hunnik E., De Boer A. H. Purification of the Fusicoccin-Binding Protein from Oat Root Plasma Membrane by Affinity Chromatography with Biotinylated Fusicoccin. Plant Physiol. 1994 Aug;105(4):1281–1288. doi: 10.1104/pp.105.4.1281. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lanfermeijer F. C., Prins HBA. Modulation of H+-ATPase Activity by Fusicoccin in Plasma Membrane Vesicles from Oat (Avena sativa L.) Roots (A Comparison of Modulation by Fusicoccin, Trypsin, and Lysophosphatidylcholine). Plant Physiol. 1994 Apr;104(4):1277–1285. doi: 10.1104/pp.104.4.1277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Markwell M. A., Haas S. M., Bieber L. L., Tolbert N. E. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem. 1978 Jun 15;87(1):206–210. doi: 10.1016/0003-2697(78)90586-9. [DOI] [PubMed] [Google Scholar]
  13. Marra M., Ballio A., Fullone M. R., Aducci P. Some Properties of a Functional Reconstituted Plasmalemma H-ATPase Activated by Fusicoccin. Plant Physiol. 1992 Mar;98(3):1029–1034. doi: 10.1104/pp.98.3.1029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Marra M., Fullone M. R., Fogliano V., Pen J., Mattei M., Masi S., Aducci P. The 30-kilodalton protein present in purified fusicoccin receptor preparations is a 14-3-3-like protein. Plant Physiol. 1994 Dec;106(4):1497–1501. doi: 10.1104/pp.106.4.1497. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Morrison D. 14-3-3: modulators of signaling proteins? Science. 1994 Oct 7;266(5182):56–57. doi: 10.1126/science.7939645. [DOI] [PubMed] [Google Scholar]
  16. Oecking C., Eckerskorn C., Weiler E. W. The fusicoccin receptor of plants is a member of the 14-3-3 superfamily of eukaryotic regulatory proteins. FEBS Lett. 1994 Sep 26;352(2):163–166. doi: 10.1016/0014-5793(94)00949-x. [DOI] [PubMed] [Google Scholar]
  17. Oecking C., Weiler E. W. Characterization and purification of the fusicoccin-binding complex from plasma membranes of Commelina communis. Eur J Biochem. 1991 Aug 1;199(3):685–689. doi: 10.1111/j.1432-1033.1991.tb16171.x. [DOI] [PubMed] [Google Scholar]
  18. Palmgren M. G., Larsson C., Sommarin M. Proteolytic activation of the plant plasma membrane H(+)-ATPase by removal of a terminal segment. J Biol Chem. 1990 Aug 15;265(23):13423–13426. [PubMed] [Google Scholar]
  19. Rasi-Caldogno F., Carnelli A., De Michelis M. I. Identification of the Plasma Membrane Ca2+-ATPase and of Its Autoinhibitory Domain. Plant Physiol. 1995 May;108(1):105–113. doi: 10.1104/pp.108.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rasi-Caldogno F., De Michelis M. I., Pugliarello M. C., Marrè E. H-pumping driven by the plasma membrane ATPase in membrane vesicles from radish: stimulation by fusicoccin. Plant Physiol. 1986 Sep;82(1):121–125. doi: 10.1104/pp.82.1.121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rasi-Caldogno F., Pugliarello M. C. Fusicoccin stimulates the H+-ATPase of plasmalemma in isolated membrane vesicles from radish. Biochem Biophys Res Commun. 1985 Nov 27;133(1):280–285. doi: 10.1016/0006-291x(85)91872-8. [DOI] [PubMed] [Google Scholar]
  22. de Boer A. H., Watson B. A., Cleland R. E. Purification and identification of the fusicoccin binding protein from oat root plasma membrane. Plant Physiol. 1989;89:250–259. doi: 10.1104/pp.89.1.250. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES