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In situ hybridization analysis of TfR2 mRNA expression in liver. Frozen sections of mouse liver were hybridized with sense (S) or antisense (AS) digoxigenin-labeled probes transcribed from the TfR2 cDNA. Signal was detected as alkaline phosphatase activity by using NBT as a substrate and appears as a dark blue product. The sections were counterstained with nuclear fast red and analyzed by light microscopy (×400).
